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KMID : 1134820200490090977
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 9 p.977 ~ p.983
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Improved Preparation of para-Nitrophenyl Maltooligosaccharides Using Cyclodextrin Glucanotransferase
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Shin Yu-Jeong
Woo Seung-Hye
Jeong Hyun-Mo
Kim Ji-Soo
Shim Jae-Hoon
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Abstract
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p-Nitrophenyl maltooligosaccharides (pNP maltooligosaccharides) are produced enzymatically by applying the disproportionation reaction of cyclodextrin glucanotransferase (CGTase) with p-nitrophenyl-α-D-glucopyranoside (α-pNPG) as acceptor molecule. In order to optimize the production of pNP maltooligosaccharides, CGTase enzyme reactions were performed under diverse conditions. pNP maltooligosaccharides were efficiently synthesized in the presence of α-pNPG and amylose at final concentrations of 3% and 0.4%, respectively. Also, by-products of the disproportionation reaction decreased dramatically in the presence of glucose oxidase (GOD). Under optimized conditions, the production yield of pNP maltooligosaccharides was about 30%
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KEYWORD
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cyclodextrin glucanotransferase, disproportionation, para-nitrophenyl maltooligosaccharides, glucose oxidase
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